Protein Identification

  • Identify 1 or 100 proteins in a fast, 8 minute assay, in almost any protein-containing sample
  • All assays performed on a Thermo Q-Exactive HF-X Orbitrap mass spectrometer
  • ID proteins present at low to sub-nanogram quantities
  • Pricing from $75 in-gel, $125 non-gel for 1 protein ID; $125 in-gel $175 non-gel for 100 protein IDs
  • No minimums
  • Typical turnaround: next week for gel-based samples, the following week for other sample types

Overview

Protein identification assays allow you to ID 1 or 100 proteins in nearly any sample type which contains protein. Via our online data portal you receive a list of proteins identified. You can drill down to the peptide level to view identified peptides, including post-translational modifications. You can even view the actual tandem mass spectra used to identify each peptide. All of the data presented online is easily downloaded.

We have leveraged the incredible speed (up to 40 sequencing events per second) and sensitivity of Thermo's newest mass spectrometer, the Q-Exactive HF-X, to offer unprecedented pricing.

Price starting at just $75 to identify a single protein in-gel, $125 non-gel. Identify 100 proteins for $125 in-gel, $175 non-gel.

Assays

Protein Identification, One Protein

  • Assay:

    Protein Identification, Top 1

  • ID: up to 1 protein group (redundant isoforms do not count to this total)
  • Specifications: UPLC-MS/MS, 15 cm column, up to 15,000 sequencing events
  • Suggested sample types: minimally complex samples where the identity of the most abundant protein needs to be determined

Protein Identification, 100 Proteins

  • Assay:

    Protein Identification, Top 100

  • ID: up to 100 protein groups (redundant isoforms do not count to this total)
  • Specifications: UPLC-MS/MS, 15 cm column, up to 15,000 sequencing events
  • Suggested sample types: minimally complex samples where the identity of the most abundant proteins needs to be determined

Sample Requirements

  • In-gel samples should:

    • contain at 1 ng or more of total protein
    • be derived from polyacrylamide gel
    • be be sized from 1 x 1 x 1 mm to a maximum of 5 x 5 x 1 mm, do not shred
    • be stained with a MS-compatible gel stain, or be unstained
    • ideally be less than 100 uL in volume, but no greater than 1.5 mL, a handling charge applies to larger volumes
  • Non-gel samples should:

    • contain at 1 ng or more of total protein
    • be in-solution or in a soluble matrix
    • be free of contaminants such as detergents or protease inhibitors, please let us know if this is not the case
    • ideally be less than 100 uL in volume, but no greater than 1.5 mL, a handling charge applies to larger volumes

Workflow

  • protein extraction and cleanup
  • enzymatic digestion with sequencing-grade trypsin
  • SPE of the digest
  • UPLC-MS/MS
  • UPLC - Thermo Easy-nLC 1200 fitted with a heated Easy-Spray column of 25 or 50 cm length
  • MS/MS - Thermo Q-Exactive HF-X quadrupole-Orbitrap mass spectrometer
  • peptide identification with Comet and Tandem, spectral library searching
  • data securely stored and accessible for one year with our basic plan

Deliverables

  • All data is hosted and delivered using our web-based data portal
  • View data reports online or download as Excel files
  • Data reports include:
  • proteins identified
  • proteins grouped by common peptide identifications, to allow isoform discrimination
  • tandem MS spectrum viewer: see fragment ion assignments for all identified peptides
  • Download raw data files (mzML format) and search result files (mzID format) -- available to plus/pro users only