Animation of Q-Exactive Orbitrap mass analyzer performing MS/MS(tandem mass spectrometry). Image courtesy of Thermo Scientific. More info
Global proteomic analysis and profiling assays allow you to ID and accurately measure hundreds to thousands of proteins in nearly any sample type which contains protein. Via our online data portal you receive a list of all proteins identified, with quantitative measurements presented across all of your samples, to easily assess differences. From there you can drill down to the peptide level to assess changes in peptide abundance, including post-translational modifications. You can even view the actual tandem mass spectra used to identify each peptide. All of the data presented online is easily downloaded.
We have leveraged the incredible speed (up to 40 sequencing events per second) and sensitivity of Thermo's newest mass spectrometer, the Q-Exactive HF-X, to offer unprecedented pricing starting at just $225 to profile up to 500 proteins. Max pricing is under $1000 - profile in excess of 6,000 proteins for only $875.
Our global proteomic analysis and profiling assays take advantage of the latest data processing methods to generate the highest quality data. Quantitation is performed by the "label-free quantitation" (LFQ) method in wide use across the industry. This method uses the "MS1" peptide peak area measurement to determine peptide abundance. The high mass accuracy (sub-5 ppm) and high mass resolution (60,000) enabled by the Orbitrap analyzer allow accurate measurements to made even in the presence of hundreds of co-eluting peptides.
The new advanced peak determination (APD) algorithm, found only in the HF-X model of the Q-Exactive line, enables almost full utilization of the instrument's sequencing capacity by improved annotation of the MS1 peaks, leading to a 40% increase in unique identifications vs. the previous instrument model (Q-Exactive HF). Multiple search algorithms (Comet and Tandem) are used for peptide identification. A spectral library is created for each individual project and searched to improve identification rates. Finally, a match-between-runs algorithm is used to impute identifications based on accurate mass and retention time values.
These assays are appropriate for a wide range of samples types, see the assay descriptions for commonly used samples. Standard sample preparation is included with these assays, including protein extraction from standard sample types. For specialized sample type assays and enrichment assays, see the sections below.
MS/MS spectra annotated with fragment ions
See each assay for assay-specific requirements, in general samples should:
Optional deliverables available at extra cost include a growing number of add-on reports: