PROTEOMIC PROFILING

Q-Exactive HF-X Orbitrap Mass Spectrometry

  • Identify and quantify thousands of proteins in a single assay
  • Characterize almost any sample type
  • Elucidate protein interactions
  • Detect and quantify post-translational modifications
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Global Proteomic Analysis and Profiling

Animation of Q-Exactive Orbitrap mass analyzer performing MS/MS. Image courtesy of Thermo Scientific.

  • Identify and quantify thousands of proteins in a fast, 20 minute assay, in almost any protein-containing sample
  • Assess differences in protein and peptide abundance between samples
  • Measure changes in isoforms and post-translational modifications
  • All assays performed on a Thermo Q-Exactive HF-X Orbitrap mass spectrometer
  • Pricing from $225, no minimums
  • Typical turnaround: next week for gel-based samples, the following week for other sample types
  • Choose from our growing list of available add-on reports for statistical tests of change, taxonomic classification and more
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Protein Identification

Protein
  • Identify 10 or 100 proteins in a fast, 8 minute assay, in almost any protein-containing sample
  • All assays performed on a Thermo Q-Exactive HF-X Orbitrap mass spectrometer
  • ID proteins present at low to sub-nanogram quantities

The Q Exactive HF-X Mass Spectrometer

"The Q Exactive HF-X Mass Spectrometer uses a high-capacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accomodates and transmits ions over a broader mass range, and a high-field Thermo Scientific™ Orbitrap™ mass analyzer."

Pricing from $75 in-gel, $125 non-gel for 1 protein ID; $125 in-gel $175 non-gel for 100 protein IDs
  • No minimums
  • Typical turnaround: next week for gel-based samples, the following week for other sample types
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Protein Sequencing and Characterization

  • Exhaustive sequencing of single, pure proteins with known sequences
  • Post-translational modification (PTM) characterization and localization
  • Characterize known amino acid substitutions, discover unknown amino acid substitutions
  • de novo sequencing of proteins with unknown sequences
  • de novo sequencing of monoclonal antibody CDR regions
  • Protein quality control monitoring of truncations, substitutions and modifications
Protein Sequencing and Characterization
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Ease-of-use

Protein identification, Top 100 Proteins Proteomics service order sample
  • Fully-integrated, one-stop online system for ordering, shipping, support and data viewing and delivery
  • Fully-automated, logged tracking of your project status from ordering, to shipment location, sample prep, mass spec and data reporting
  • Pay by credit card, PO support, share quotes and invoices, easily send payment link to third-party payor
  • Order- and project-linked support for fast administrative or technical support that doesn't get lost in email threads
  • Request our shipping kit, or just get a shipping label to use with your own box
  • Online data viewing, or download to your desktop; access to raw data and search output files with paid plans
Shipping info
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Data Processing

  • Data is processed by assay-specific workflows on AWS, which means fast processing and secure, reliable data storage
  • Order add-on reports now or later, based on your sample results
  • Support for custom data processing workflows:
    • –   request specific changes to one of our workflows
    • –   develop a completely custom workflow for your samples
    • –   process your own data files with our workflows, or with custom workflows we develop for you
Fully-automated data processing and report delivery following assay completion
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Reports

Proteomic service specific reports for LFQ assay Taxonomy report
  • High quality, use case-specific reports for every assay, designed to make the relevant data easily accessible
  • A selection of optional add-on reports for specialized data analysis, including statistical assessment of sample-wise comparisons, taxonomic classifications and more
  • Support for custom reports for paid plans:
    • –   anything from simple table alterations to publication-quality figures
    • –   Certificates of Analysis
    • –   highly-customized write-ups for quality control, sample validation, etc.
Proteins and peptides identified in the samples in the project
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Publications

Our work has contributed to publications in leading scientific journals
Datta, Poppy, Allamargot, Chantal, Hudson, Joseph S., Andersen, Emily K., Bhattarai, Sajag, Drack, Arlene V., Sheffield, Val C. Seo, Seongjin, 'Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.'
Bronisz, Agnieszka, Wang, Yan, Nowicki, Michal O., Peruzzi, Pierpaolo, Ansari, Khairul I., Ogawa, Daisuke, Balaj, Leonora, De Rienzo, Gianluca, Mineo, Marco, Nakano, Ichiro, Ostrowski, Michael C., Hochberg, Fred, Weissleder, Ralph, Lawler, Sean E., Chiocca, E. Antonio Godlewski, Jakub, 'Extracellular vesicles modulate the glioblastoma microenvironment via a tumor suppression signaling network directed by miR-1.'
Rialdi, A., Campisi, L., Zhao, N., Lagda, A. C., Pietzsch, C., Ho, J. S. Y., Martinez-Gil, L., Fenouil, R., Chen, X., Edwards, M., Metreveli, G., Jordan, S., Peralta, Z., Munoz-Fontela, C., Bouvier, N., Merad, M., Jin, J., Weirauch, M., Heinz, S., Benner, C., van Bakel, H., Basler, C., Garcia-Sastre, A., Bukreyev, A. Marazzi, I., 'Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation'
Burtnick, Mary N., Brett, Paul J. DeShazer, David, 'Proteomic Analysis of the Burkholderia pseudomallei Type II Secretome Reveals Hydrolytic Enzymes, Novel Proteins, and the Deubiquitinase TssM.'
Meckes, David G., Gunawardena, Harsha P., Dekroon, Robert M., Heaton, Phillip R., Edwards, Rachel H., Ozgur, Sezgin, Griffith, Jack D., Damania, Blossom Raab-Traub, Nancy, 'Modulation of B-cell exosome proteins by gamma herpesvirus infection.'
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Our Customers

Serving scientists since 2008
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